Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
High expression yield and high volumetric productivity of fermentations employing recombinant E. coli need special processing. For recombinat protein production two strategies can beapplied. One is to carry out one step fermentation with simultaneous gene expression andelimination of vector-less segregants. The other is to carry out two step fermentation, in whichthe first step is the cell biomass production without gene expression followed by the inductionof gene expression in the second step. In this review different methods of controlling of recombinant gene expression, such as antibiotic pressure, specific mutant application, immobilizedcell bioreactors, selective cell recyrculation, chemical regulations, the use of temperature andoxygen sensitive promoters are presented.
Biotechnologia, vol.28, 1 (1995)-.
0860-7796 ; oai:rcin.org.pl:148038 ; IChB B-24
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 22, 2020
Nov 22, 2020
170
https://rcin.org.pl./publication/183578
Edition name | Date |
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Czynniki determinujące ekspresję zrekombinowanych genów in vitro u Escherichia coli. Część II - Hodowla w bioreaktorze | Nov 22, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Grajek, Włodzimierz
Grajek, Włodzimierz
Grajek, Włodzimierz
Nowak, Jacek K.