Subtitle:

Molecular Methods for Detection, Identification and Characterization of Fusarium

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Pathogenic fungi of the genus Fusarium form a diverse, cosmopolitan groupofspecies. Their importance is significant due to the fact that they are responsible for numerous storage rots, plant diseases and human and animal diseasecaused by Fusarium toxic secondary metabolites. By reason of great extent ofvariability and plasticity of Fusarium easily accomodating to changing environmental condition, this genus comprises ofspecies that remain very challengingfor systematics. Since the reliable and simple detection methods are essentialto avoid the losses and health risks, molecular biology methos have been recently implemented for the analysis of Fusarium populations. In this paper, wepresent a review ofselected PCR-based techniques applicable for diagnostic andtaxonomic purposes. The RAPD analysis, followed by SCAR-PCR and RFLP methodto study fungal rDNA are presented. A number of primer sequences useful forthe above methods are also listed.

Relation:

Biotechnologia, vol.58, 3 (2002)-.

Volume:

58

Issue:

3

Start page:

165

End page:

177

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:137919 ; IChB B-54

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open