Subtitle:

RNAi, PTGS and quelling - three variations on one subject?

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In 1990, it was already discovered that plant transformation with a transgenecontaining its homologue in the plant nuclear genome is able to promote silencing of both the transgene and the homologous, endogenous gene. The phenomenon was named posttranscriptional gene silencing (PTGS) or co-suppression.The same results were obtained when a transgene was introduced into the nuclear genome of fungus Neurospora crassa. This process was termed quelling. In1998, RNAi (RNA interference) was discovered in the Caenorhabditis elegansworm. Specific gene silencing occurred after the introduction into the wormcells of double stranded RNA with sequence complementarity to the endogenous gene. It was shown that RNAi operates at the stage ofthe mature mRNA inthe cytoplasm. dsRNAs are converted into siRNAs (small interfering RNAs) dueto the Dicer enzyme activity. siRNAs are incorporated into the RISC (RNA - induced silencing complex). Active RISC promotes specific mRNA degradation.RNAi/PTGS/quelling processes show many mechanistic similarities, but they alsodiffer in some details. All of them represent an ancient mechanism that probably evolved to protect eukaryotic cells against invasive forms of nucleic acidslike viruses, transposons, and others.

Relation:

Biotechnologia, vol.61, 2 (2003)-.

Volume:

61

Issue:

2

Start page:

54

End page:

66

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:135561 ; IChB B-57

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open