Subtitle:

Transgene expression and gene silencing in cereals

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Genetic transformation of cereal crops is a powerful research tool for analysis of gene function and varietal improvement. Application of the method is possible when the expression of introduced transgene is on the desired level and stable over several generations. The production of transgenic cereals was mainly performed by microprojectile bombardment. However, some advance was also achieved by application of Agrobacterium-med'iated transformation. For rice, which is the cereal model species, this method is routinely used, while for many others, especially polyploids, it has been developed very recently and only in a few laboratories. We still lack the knowledge whether the main features of Agro-mediated transformation (i.e. integration of one or few copies usually not rearranged and well defined transgene cassette) influence the transgene expression in polyploid cereal species. This review discusses known mechanisms possibly involved in transgene silencing, using both transformation methods. Part of the discussion is focused on transgene expression / silencing in relation to large genomes of polyploid cereals.

Relation:

Biotechnologia, vol.75, 4 (2006)-.

Volume:

75

Issue:

4

Start page:

168

End page:

180

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:87238 ; IChB B-71

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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