Isoenergetic microarrays, a new method to study the structure of RNA
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
RNA is one of the most important molecule essential for proper functioning of the cell. However, there are a growing number of RNAs for which biological function and structure are not known. Isoenergetic microarrays have been designed and apply as a new method to study the structure and interactions of RNAs. Isoenergetic microarrays are built with short modified RNA probes. The 2’-0-methylated and LNA-modified oligomer probes have been designed in such a way, that the interaction of each probe with its complementary single stranded fragment of target RNA has comparable thermodynamic stability (is isoenergetic). This method was tested and optimized on several native RNAs.
Biotechnologia, vol.83, 4 (2008)-.
0860-7796 ; IChB-78 ; oai:rcin.org.pl:73257
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Jun 18, 2019
279
https://rcin.org.pl./publication/95945
Edition name | Date |
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Mikromacierze izoenergetyczne, nowa metoda badania struktury RNA | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan