Subtitle:

Methodological aspects of proteomic analyses involving two-dimensional electrophoresis and mass spectrometry

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Description:

prace przeglądowe

Abstract:

Proteomics is a scientific discipline that focuses on the large-scale study of proteins, particularly their structures, functions and interactions. The proteome is the full complement of proteins expressed by genome. During the last decade, thanks to subset of proteomic techniques and workflows, it has been possible to identify: diagnostic protein biomarkers, potential therapeutic targets and also biotechnologically important plant proteins, like those that give a plant resistance to drought. Over the last years, proteomics has generated a relatively large number of reviews on technical aspects and concepts. Although total automation and reproducibility are possible, the protocols of protein isolation and separation are sample specific. Moreover, most of them address the development of optimal sample preparation protocols of mammalian cells and tissue. In contrast, plant proteomics is still in its infancy, probably because plant material is very recalcitrant. The objective of this review is to pay attention to some methodological aspects of proteomic analyses using 2DE and mass spectrometry, especially in the case of plant material.

Relation:

2009 ; Biotechnologia, vol.85, 2 (2009)-.

Volume:

85

Issue:

2

Start page:

7

End page:

26

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:72110

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2009

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty