Subtitle:

Niedokrwienie hamuje neurony GABAergiczne w jądrze siatkowatym wzgórza u szczurów. Badania immunocytochemiczne

Publisher:

Medical Publishing House

Place of publishing:

Warsaw

Type of object:

Journal/Article

Abstract:

GABA-immunoreactivity in the thalamic reticular nucleus was studied in rats subjected to 10 min global cerebral ischemia, due to experimentally induced cardiac arrest. The studies were performed in different postischemic periods (10 min, 1 h and 24 h after ischemia) with postembedding immuno-gold technique for electron microscopy, applying antisera raised against protein-gamma amino butyric acid-conjugates. Transient reduction of gold particles content, indicating GABA appearance and distribution in neuronal perikarya and synaptic terminals, was noticed 10 min and 1 h after ischemia. Reduction of immunoreactivity accompanied ultrastructural abnormalities involving both neurons and synapses and taking the form of severe swelling and disorganization of organelles arrangement. Immunocytochemical abnormalities concerning neuronal perikarya appeared earlier and were more severe. At 24h after ischemia immunoreactivity of most of the neurons and synapses was similar to this in normal control animals. Morphologically unchanged asymmetric synapses were present in all experimental groups. The presented data confirm high vulnerability of GABAergic neuronal population of the thalamic reticular nucleus to ischemia and suggest transient nature of postischemic GABAergic insufficiency.

Relation:

Folia Neuropathologica

Volume:

32

Issue:

3

Start page:

139

End page:

149

Detailed Resource Type:

Article

Format:

pdf

Resource Identifier:

oai:rcin.org.pl:71851

Language:

eng

Language of abstract:

eng ; pol

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open