Institutional creator:

Instytut Medycyny Doświadczalnej i Klinicznej im. Mirosława Mossakowskiego PAN

Contributor:

Sarnowska, Anna (Promotor) ; Zychowicz, Marzena (Promotor pomocniczy)

Publisher:

Instytut Medycyny Doswiadczalnej i Klinicznej im. Miroslawa Mossakowskiego PAN

Place of publishing:

Warszawa

Degree name:

PhD

Level of degree:

2

Degree discipline :

medical sciences

Degree grantor:

Instytut Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN

Type of object:

Praca dyplomowa

Abstract:

Stem cells (SCs) are the basis of cellular therapies, widely used in regenerative medicine. Mesenchymal stem/stromal cells (MSCs) are one of frequently studied adults SCs group, classified as multipotent, due to differentiational potential toward mesodermal. However, researchers reported the possibility of MSCs differentiating into cells derived from other germ layers, such as neurons (ectoderm) or hepatocytes (endoderm). Furthermore, under specific environmental conditions, MSCs exhibit the expression of genes related with pluripotent stem cells (capable to differentiate toward any cell in human body). Hereby, this dissertation attempted a verification whether MSCs could contain subpopulation exhibiting pluripotent characteristics. Conducted experiments examined different factors for separation of cells possessing undifferentiated potential, that exhibit high proliferation and self-renewal potentials, elevated pluripotent genes expression (SOX2, OCT3/4, NANOG) and differentiate toward cells from all three embryonic germ layers. MSCs were isolated from Wharton Jelly (WJ-MSCs) – a part of umbilical cord which is a commonly used MSCs source. During the first stage of doctoral research, WJ-MSCs, usually propagated in adherent 2D conditions, were transferred to 3D conditions, by modification of culture method proposed for neural stem cells. During the second stage of doctoral research WJ-MSC positive for SSEA-4 were isolated and characterized as a theoretic population with a wider potential. In summary, presence of pluripotent-like subpopulation in WJ-MSC was not confirmed by chosen methods. However, observed results indicated a wide heterogeneity and diversity of subpopulations constituting to MSCs. Selection and detailed analysis of specific cell groups demand further research to relate their therapeutic applications.

Detailed Resource Type:

Praca doktorska

Format:

pdf

Resource Identifier:

oai:rcin.org.pl:242399

Source:

IMDiK PAN, ZS 436 ; click here to follow the link

Language:

pol.

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Access:

Open