Institutional creator:

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego PAN

Contributor:

Łukasiuk, Katarzyna (1965– ) : Supervisor

Publisher:

Nencki Institute of Experimental Biology PAS

Place of publishing:

Warsaw

Description:

X, 127 pages : illustrations ; 30 cm ; Bibliography ; Summary in Polish

Degree name:

PhD in Biological Sciences

Degree discipline :

biological sciences

Degree grantor:

Nencki Institute of Experimental Biology PAS ; degree obtained on 12/04/2024

Type of object:

Thesis

Abstract:

While the relationship between epilepsy and circadian dysregulation is known, we know very little about circadian oscillations of the transcription factors which are responsible from modulating the gene expression in health and disease pathologies. This study aims to characterise circadian dynamics of one of the identified and prominent transcription factors in epilepsy – the Zbtb14. In pursuit of this objective, protein rhythmicity of the Zbtb14 is observed in the ventral and dorsal hippocampus and the somatosensory cortex using immunochemistry, and in cytoplasmic and nuclear protein extracts of the hippocampus using western blot. The downregulated genes with ZF5 motif in their promoters identified in an in vitro model of epileptiform discharges is characterised. Zbtb14 protein expression is investigated in two time points in an in vivo model of epilepsy model. The study showed that Zbtb14 protein only has a rhythmic expression in the ventral hippocampus but not in the dorsal hippocampus and the somatosensory cortex. Additionally, the cytoplasmic and nuclear dynamics of the Zbtb14 protein are different. I identified the downregulated genes in in vitro model of epileptiform discharges are mainly responsible from synaptic plasticity and transmission. Furthermore, the epilepsy pathology affected the Zbtb14 transcription factor expression in a time-dependent manner. My research shows that the studies on circadian regulations of the transcription factors can be beneficial target to unravel the disease pathologies and potential therapeutics.

Detailed Resource Type:

PhD Dissertations

Resource Identifier:

oai:rcin.org.pl:241157

Source:

IBD PAN, call no. 20303

Language:

eng

Language of abstract:

pol

Terms of use:

Copyright-protected material. May be used within the limits of statutory user freedoms

Copyright holder:

Publication made available with the written permission of the author

Digitizing institution:

Nencki Institute of Experimental Biology of the Polish Academy of Sciences

Original in:

Library of the Nencki Institute of Experimental Biology PAS

Access:

Open