Twórca instytucjonalny:

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego PAN

Współtwórca:

Włodarczyk, Jakub : Supervisor ; Bijata, Monika : Assistant supervisor

Wydawca:

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego PAN

Miejsce wydania:

Warszawa

Opis:

117 pages : illustrations ; 30 cm ; Bibliography ; Summary in English

Uzyskany tytuł:

PhD in Biological Sciences

Dyscyplina :

Biological Sciences

Instytucja nadająca tytuł:

Nencki Institute of Experimental Biology PAS ; degree obtained: 14.04.2023

Typ obiektu:

Thesis

Abstrakt:

Depression is a global medical problem frequently leading to suicides. It is assumed that depressive symptoms occur as a result of aberrant excitatory synaptic plasticity developed after chronic stress. Synaptic plasticity is an ability of neurons to modulate the strength of synaptic connections manifested by structural alterations of dendritic spines on which excitatory synapses are located. Recently discovered activation of the serotonin type 7 receptor (5-HT7R)-dependent signaling pathway leads to aberrant structural and functional synaptic plasticity in the hippocampal neurons in vitro. However, whether this signaling pathway exists in vivo or not and what its role is, it has not yet been investigated.Using a combination of behavioral, biochemical, and imaging methods, it has been demonstrated that the 5-HT7R-dependent signaling pathway is activated in the murine hippocampus and underlies the depressive-like phenotype. Additionally, the results confirmed that the activation of the 5-HT7R-dependent signaling pathway is associated with the structural remodeling of dendritic spines of anhedonic animals. The stress-resilient animals did not display the aforementioned biochemical and structural alterations indicating the specificity of the obtained results. Moreover, the implementation of the chronic unpredictable stress procedure with specific silencing of htr7 gene expression in the CA1 hippocampal subregion enabled to discover that silencing of 5-HT7R in the hippocampus is sufficient to prevent the development of anhedonia.The obtained results point out a crucial role of 5-HT7R in the pathogenesis of depressive-like behavior and the dendritic spine structure as a possible decisive molecular target for promoting stress resilience in future pharmacotherapies.

Szczegółowy typ zasobu:

PhD Dissertations

Identyfikator zasobu:

oai:rcin.org.pl:238068

Źródło:

IBD PAN, call no. 20185

Język:

pol

Język streszczenia:

eng

Prawa:

Rights Reserved - Free Access

Zasady wykorzystania:

Copyright-protected material. May be used within the limits of statutory user freedoms

Właściciel praw autorskich:

Publication made available with the written permission of the author

Digitalizacja:

Nencki Institute of Experimental Biology of the Polish Academy of Sciences

Lokalizacja oryginału:

Library of the Nencki Institute of Experimental Biology PAS

Dostęp:

Open