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Crystallization of nucleic acids
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Crystallization is the most important and challenging step in the determination of the structure of biomolecules and macromolecules by diffraction methods.Macromolecules are extremely complex physical-chemical system whose properties vary as afunction of many environmental influences such as temperature, pH, ionic strength and solventcomposition. Therefore, a number of factors influencing macromolecule crystal growth ought tobe considered. Despite of considerable interest in the approaches allowing for continuous monitoring and setting of crystal growth conditions, in practice, numerous trials ought to be performedin order to obtain appropriate crystals characterized by their optimal geometrical parameters,crystallographic symmetry, unit cell and, most importantly, their high resolution.The vapour diffusion and the microdialysis methods are discussed as the most widely usedcrystallization methods of macromolecules.
Biotechnologia, vol.23, 4 (1993)-.
0860-7796 ; oai:rcin.org.pl:149099 ; IChB B-19
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 27, 2020
Nov 27, 2020
452
https://rcin.org.pl./publication/184603
Edition name | Date |
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Krystalizacja kwasów nukleinowych | Nov 27, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan