Subtitle:

Application of transfer onto PVDF and nitrocellulose membranes in the purification and structural analyses of proteins

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Replicas of SDS-PAGE gels widen the range of application of electrophoresis to the studiesof diverse biological properties of proteins as well as to the elucidation of their structures. Mostoften proteins are transferred to membranes made of pol3rvinylidene difluoride (PVDF) or nitrocellulose. Usually, a “semi-dry” blotting apparatus is used for electrophoretic transfer. SDS-PAGEgel replicas are used for the studies of these biological properties which cannot be studieddirectly on polyacrylamide gels. The major applications of this technique is in the immunoblottingwhich allows for the antigen-antibody reaction on the membrane. This reaction, after necessaryamplification, is visualized with a variety of reagents. Protein microsequencing is another important application of SDS-PAGE gel replicas. In this case, a replica is stained with a generalprotein stain, and a band of interest is excised and used to obtain partial or complete aminoacid sequence in an automatic sequencer.

Relation:

Biotechnologia, vol.32, 1 (1996)-.

Volume:

32

Issue:

1

Start page:

129

End page:

150

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:147320 ; IChB B-28

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open