Subtitle:

In Vitro Culture of Cereal Microspores

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

The development of microspore culture methods in the Poaceae family hasreceived considerable attention in recent years. Isolated microspore culture canbe induced in vitro to switch their development from gametophytic to a sporophytic patway, resulting in embryoid or callus formation. Different stresseslike cold or heat shock and nitrogen starvation have been identified as the major trigger in inducing microspore embryogenesis. Microspore culture appearsto be a promising tool for future production of double haploids in cereals. Isolated microspore culture has several advantages over anther culture in geneticmanipulation and haploid study, such as: direct observation of microspore development, unique possibility to study plant embryogenesis, easier in vitro selection and mutation, easier transformation of single cells. Moreover, isolatedmicrospores are the most efficient way of double haploid regeneration. Manyfactors such as genotypes, physiological status of donor plants, stage of microspores development, pretreatment of anthers or spikes, method of microsporesisolation, culture media, nurse culture and culture conditions, have a great influence on microspore culture. These and other problems concerning in vitroculture of isolated microspore are discussed in this review.

Relation:

Biotechnologia, vol.49, 2 (2000)-.

Volume:

49

Issue:

2

Start page:

142

End page:

160

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:141527 ; IChB B-45

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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