Subtitle:

The influence of methylene blue and methylene green on the effectiveness of photooxidation of porcine collagen

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Photooxidation is a method of tissue fixation in the construction ofbioprostheses. This process leads to the tissue protein crosslinking under lightand dye. In the present study, the influence of methylene blue and methylenegreen on the effectiveness of pulmonary valve and aorta collagen modificationwas evaluated on the basis of photooxidized tissues sensitivity to pepsin digestion. The components of tissue hydrolysates were characterized electrophoretically. The tissues photooxidized in the presence of employing dyes showed increased resistance to enzymatic degradation in comparison to the unfixed ones.However, methylene green was more effective in valve and aorta collagencrosslinking. Hydrolysates of the tissues fixed in the presence of mentioned dyedisplayed decrease in collagen a chains and their aggregates’ content.

Relation:

Biotechnologia, vol.61, 2 (2003)-.

Volume:

61

Issue:

2

Start page:

290

End page:

302

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:135756 ; IChB B-57

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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