Subtitle:

Short interfering RNAs as tools for sequence-specific gene silencing

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In diverse eukaryotes, dsRNA triggers the destruction of mRNA sharing thesame sequence as the dsRNA in the process called RNAi. The guides for sequence-specific degradation of mRNA are 21 nt short interfering RNAs (siRNAs).Synthetic siRNAs can efficiently mediate RNAi, but a drawback of RNAi is itstransient nature as a result of the limited availability and stability of syntheticoligonucleotides. Recently, several groups reported the construction of expression plasmid vectors that mediate the production of siRNAs under control ofPol III promoters. These vectors allow the continued expression of siRNAs in thecells resulting in persistent and specific suppression of target genes. The retroviralsiRNA expressing system allows for stable inactivation of the genes in primarycells or living organisms.

Relation:

Biotechnologia, vol.61, 2 (2003)-.

Volume:

61

Issue:

2

Start page:

84

End page:

103

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:135618 ; IChB B-57

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open