Subtitle:

Instability of ploidy level during regeneration of transformed andnon-transformed tobacco (Nicotiana tabacum L.)

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In plant tissue cultures, somaclonal variation is often observed. It can be aneffect of the changes in the individual chromosome number or in the ploidylevel. Flow cytometry, a fast and accurate method for the estimation of the nuclear DNA content, can be applied to study these changes. The DNA content indifferentiated tissues of Nicotiana tabacum cultured in vitro was estimated usingPartec CCA flow cytometer, starting from explant, through callus, up to regenerated shoots. The explant constituted stem segments of N. tabacum plants,non-transformed and transformed with gfp gene. Flow cytometric analysisshowed differences in the proportion of 2C, 4C, 8C and 16C cells in plant tissuein different culture stages. Among the regenerated plantlets originated fromnon-transformed and transformed plants, diploid, tetraploid and mixoploidforms were observed. The transformation did not influence the share of cellsrepresenting different ploidy levels in the investigated plant material.

Relation:

Biotechnologia, vol.62, 3 (2003)-.

Volume:

62

Issue:

3

Start page:

260

End page:

266

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:133333 ; IChB B-58

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open