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Cryopreservation of cell suspensions of selected Gentiana
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Cryopreservation is recognised as the most efficient method of plant genome preservation and embryogenic potential of plant in vitro cultures withnon-changed status. Because of the risk of biological degradation of tissue during freezing treatment and it various sensibility on the chilling stress, the individual treatment with cryoprotectance and parameters of the temperature decreasing is highly required.In this presentation, following subjects will be discussed: 1) description ofsuspension cultures of various gentians, 2) cell pretreatments for surviving thestress of low temperature, 3) rearrangement of cell cytoplasm because of highsucrose concentrations, 4) the role of sucrose in the development of freezingtolerance and surviving of gentaiana suspensions, 5) the effect of strongplasmolysis induced by 3 M glucose.
Biotechnologia, vol.62, 3 (2003)-.
0860-7796 ; oai:rcin.org.pl:133109 ; IChB B-58
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Oct 2, 2020
Jul 21, 2020
159
https://rcin.org.pl./publication/167735
Edition name | Date |
---|---|
Krioprezerwacja zawiesin komórkowych wybranych gatunkówz rodzaju Gentiana | Oct 2, 2020 |
Tyczewska, Agata Bąkowska- Żywicka, Kamila
Pieczyński, Marcin Bielewicz, Dawid Dolata, Jakub Szweykowska-Kulińska, Zofia