Subtitle:

Application of modern methods for plant material studies of Gentiana kurroo (Royle) somatic embryogenesis

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Nowadays somatic embryogenesis is being investigated with special attention paid to the identification of genes directly involved in triggering of cell competence and development of ontogenic stages. In order to trace successive cell divisions that lead to the formation of the globular structures, modern scanning and electron microscopy methods are applied. Plantlets, which come from somatic embryogenesis process, should be exactly the same as mother plants but in vitro culture conditions may induce many disturbances, which could be lasting (hereditary) or only transitory. These changes are usually called soma- clonal variation and could be observed on different levels of the plant organization. To investigate this kind of variations both of the genetic and epigenetic types, specially designed molecular systems are needed. Here, we describe induction of somatic embryos from several explants of G. kurroo. In order to evaluate the particular ontogenic stages of somatic embryos and variability of regenerated plants, following methods were applied: light microscopy, scanning and transmission electron microscopy, 2D protein electrophoresis, flow cytometry of DNA content in the cell nucleus, cytogenetic analysis of chromosome number and molecular analysis with the use of AFLP.

Relation:

Biotechnologia, vol.75, 4 (2006)-.

Volume:

75

Issue:

4

Start page:

95

End page:

106

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:87231 ; IChB B-71

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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