Title:

Monoclonal antibodies against isoprenoid compounds

Subtitle:

Monoclonal antibodies against isoprenoid compounds

Creator:

Wanke, Małgorzata ; Porębska, Anna ; Wałajtys-Rode, Elżbieta

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2001

Subject and Keywords:

biotechnology

Abstract:

Monoclonal antibodies against isoprenoid compounds were obtained by invivo immunization of Balb/c mice with UQ-10 (ubiquinone-10) incorporated intocholesterol rich particles containing metylated bovine serum albumin(CRP-MBSA) in order to induce a strong immune response. The specificity of antibodies was evaluated by a modified dot-blot technique using Hybond-N +membrane (Amersham). Selected antibodies 1II-5 (lgG3), lV-3 (IgG2b) and VII-11(IgM) were capable of recognizing different isoprenoid compounds: ubiquinone,plastoquinone, solanesol, dolichol, p-sistosterol but did not react withphospholipids, triacylglycerols or fatty acids. These antibodies may be used inimmunoblotting for identifying isoprenoids (e.g. ubiquinone) in partially purified plant extracts after TLC chromatography on glass RP-18 plates (Merck).Immunodetection ofisoprenoids was performed using secondary goat anti-miceimmunoglobin antibodies conjugated with biotin, extravidine-AP and BCIP/NBTas substrate.

Relation:

Biotechnologia, vol.54, 3 (2001)-.

Volume:

54

Issue:

3

Start page:

169

End page:

177

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-50

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open