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Construction of plasmid bicistronic vectors and their application for in vitro transfection
Subtitle:Construction of plasmid bicistronic vectors and their application for in vitro transfection
Creator:Kucharzewska, Paulina ; Zagórska, Anna ; Leja, Justyna ; Jaźwa, Agnieszka ; Gozdecka, Małgorzata ; Józkowicz, Alicja ; Dulak, Józef
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:Internal ribosome entry site (IRES) sequences, which stand for the basic element of cap 5’-independent translation, are currently widely used to coexpress heterologous genes from one plasmid. In this study construction of four bicistronic plasmids containing IRES and application of these vectors for transfection of in vitro cultured cells were described. The obtained data show that constructed bicistronic plasmids are very efficient in vitro in terms of simultaneous expression of fibroblast growth factor-4 (FGF-4) and vascular endothelial growth factor (VEGF) or one of these factors and green fluorescent protein (GFP) from one plasmid. Interestingly, expression of two genes, although simultaneous, is not equal. It has turned out that IRES-dependent mRNA translation is less efficient than cap 5’-dependent translation of the first gene, which should be taken into account during construction of bicistronic plasmids.
Relation:Biotechnologia, vol.78, 3 (2007)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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