Metadata language
Potato and tobacco cultivars transformation towards potato virus Y resistance
Subtitle:Potato and tobacco cultivars transformation towards potato virus Y resistance
Creator:Chachulska, Anna Maria ; Chrzanowska, Mirosława ; Flis, Bogdan ; Krzymowska, Magdalena ; Lipska-Dwuźnik, Anna ; Robaglia, Christophe ; Zagórski, Włodzimierz
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:In recent years a new subgroup of necrotic potato virus Y (PVY) isolates has spread in thePolish fields. To counter this infection, the PVY resistance was introduced into selected potatoand tobaeeo cultivars. Plants were transformed by agroinfection with pROK2 derived binaryplasmids, carrying an appropriate fragment of the PVY genome (patent applied for). Kanamycinresistant plants were screened by PCR for the presence of PVY cDNA inserts, positive transformants were tested for virus resistance by inoculation with sap from infected plants, followed byobservations of disease symptoms and virus accumulation analysis. Several resistant potato andtobacco clones were identified and their phenotypes were preliminarily characterised.
Relation:Biotechnologia, vol.39, 4 (1997)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Projects co-financed by: Access: