Title:

Development of an automated yeast identification system by the application of PCR technique: a comparison of Inter-LINE PCR with reference methods

Subtitle:

Development of an automated yeast identification system by the application of PCR technique: a comparison of Inter-LINE PCR with reference methods

Creator:

Salek, Anna Teresa

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Date issued/created:

2001

Subject and Keywords:

biotechnologia

Abstract:

In the Inter-LINE (IL) PCR method, oligonucleotides GF, GR and 01, whichoriginated from mammalian cells led to highly reproducible patterns of amplified template DNA, based on the consensus of LlNE-sequences. These were usedfor the genomic fingerprinting of about 80 strains of yeast, consisting of 30 species from 13 genera. The IL-PCR technique using the above primers is describedand compared to reference methods such as Arbitrarily Primed-PCR and PulseField Gel Electrophoresis (PFGE).A comparison of the two PCR variants was performed using suitable numbers of digitized PCR-fmgerprints. A database for an automated yeast identification system is proposed.

Relation:

Biotechnologia, vol.55, 4 (2001)-.

Volume:

55

Issue:

4

Start page:

122

End page:

134

Resource type:

Tekst

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-50

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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