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Produkcja wektorów adenowirusowych pierwszej generacji - optymalizacja metody
Subtitle:Production of adenoviral vectors of the first generation - optimization of the method
Creator:Stopa, Magdalena ; Jaźwa, Agnieszka ; Mleczko, Katarzyna ; Dulak, Józef ; Józkowicz, Alicja
Publisher:Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN
Date issued/created: Subject and Keywords: Abstract:The paper presents the production of adenoviral vectors (AdV) containing P-galactosidase (Adp-gal), from the transfer of recombinant viral DNA into packing cell line (HEK293) to the titration of viral particles. Optimisation of the methods (preparation of DNA for transfection, adsorption time during the infection of cells, amount of serum in the medium, time-point of vector isolation) enables obtaining a titer of up to 10'° lU/mL. Adp-gal were titrated with several methods, with P-gal in situ staining used as a reference. We found that the most suitable titration method of the vectors containing other than reporter genes was the Rapid Titer ELISA kit™.
Relation:Biotechnologia, vol.78, 3 (2007)-.
Volume: Issue: Start page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
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