Title:

Wykorzystanie androgenezy w kulturach pylnikowych do otrzymywania homozygotycznych roślin marchwi

Subtitle:

Obtaining homozygous carrot plants with the aid of androgenesis in anther cultures

Creator:

Górecka, Krystyna ; Krzyżanowska, Dorota ; Kiszczak, Waldemar ; Kowalska, Urszula ; Górecki, Ryszard

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Date issued/created:

2008

Subject and Keywords:

biotechnologia

Abstract:

Experiments with anther cultures of 22 carrot cultivars were carried out to study the effect of various factors on the effectiveness of embryogenesis in these cultures. The factors included: the stage of microsporogenesis, genotype, training of donor plants and their growth conditions. A modified B5 medium (Gamborg, et al. 1968) containing 500 mg L’ glutamine, 100 mg L ’ serine, 0.1 mg L ' of 2,4D, 0.1 mg L ’ NAA, 100 g L ’ sucrose and 6.5 g L ’ agar were used to induce androgenesis. Regeneration was carried out on MS media and B5 with reduced concentration of sucrose at 20 g L ’ without aminoacids and hormones or with small amount of hormones. Substrates that were a mixture of various components, such as peat, sand, mineral wool and charcoal, were used for adaptation. Ploidy of the obtained plants was determined by cytometry method. Homozygosity of the plants was established using two isoenzymatic systems: PGl — phosphoglucose isomerase, and /\AT — aspartate aminotransferase. Anatomical studies of embryogenesis during antlier cultures were also carried out to confirm the androgenetic origin of embryos.

Relation:

Biotechnologia, vol.81, 2 (2008)-.

Volume:

81

Issue:

2

Start page:

142

End page:

152

Resource type:

Tekst

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-76

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2008

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

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