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Cryopreservation as a method to facilitate presenation of life capacity of plant cells and tissues
Subtitle:Cryopreservation as a method to facilitate presenation of life capacity of plant cells and tissues
Creator: Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:This paper presents a review of fundamental aspects of plant cryopreservation. Liquid nitrogen has several advantages over storage of vegetatively propagated material under normal low-temperature in vitro culture and could also help in preserving genetic biodiversity. Development of efficient cryopreservation protocols based on the induction of tolerance to freezing and/or desiccation is also discussed. Cold and/or preculture acclimatization leads to ultra- structural, physiological and molecular changes in cells and they are important for improving viability after cryopreservation. The application of vitrification-based procedures and ultra-fast freezing/thawing rates could be effective and reliable for wide variety of plant species/tissues and relatively genotype independent. Majority of papers demonstrate that the liquid nitrogen allows high viability rates and re-growth without a loss of biosynthetic capacity. Up to now, there has been no clear evidence of morphological, cytological or genetic alterations due to cryopreservation.
Relation:Biotechnologia, vol.81, 2 (2008)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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