Title:

Application of real-time PCR technique for validation of microarray experiment

Subtitle:

Application of real-time PCR technique for validation of microarray experiment

Creator:

Ciesielska, Agnieszka ; Sikorski, Michał M.

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2008

Subject and Keywords:

biotechnology

Abstract:

Microarray technology has recently reached the mainstream. It is widely applied in many biological fields of studies. Despite of its uncontested advantages microarray results have to be validated using independent methodology. For this purpose real-time PCR platform has become the most popular method of choice. The ability of PCR product measurement after each cycle is connected here with wide dynamic range of detection. This review is a summary of our knowledge on the technical issue of quantitative PCR utilization in microarray results confirmation.

Relation:

Biotechnologia, vol.83, 4 (2008)-.

Volume:

83

Issue:

4

Start page:

88

End page:

100

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB-78

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open