Title:

Ultrastructural study of PECAM-1/CD 31 expression in the developing mouse blood-brain barrier with the Application of a pre-embedding technique

Subtitle:

Badania ultrastrukturalne ekspresji PECAM-1/CD31 rozwijającej się bariery krew-mózg u myszy, z zastosowaniem techniki przedzatopieniowej

Creator:

Lossinsky, AS. ; Wiśniewski, HM. ; Dąmbska, Maria ; Mossakowski, Mirosław Jan (1929–2001)

Publisher:

Severus Publishing House

Place of publishing:

Warsaw

Date issued/created:

1997

Type of object:

Journal/Article

Subject and Keywords:

PECAM-1/CD31 ; Blood-brain barrier ; adhesion molecules ; developmental blood-brain barrier

Abstract:

The aim of this study was to investigate the time of expression of the adhesion molecule platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) in the developing mouse blood-brain barrier (BBB). Ultrastructural studies employing a preembedding technique described here in demonstrate that PECAM-1 is initially expressed on the luminal and abluminal endothelial cell surfaces in the newborn animals. This adhesion molecule expression appears to increase in intensity at 7-10 days post partum and then decreases to a weak labelling of the luminal endothelial cell surfaces at two weeks after birth. Our results present immunocytochemical detection of active angiogenesis during early brain development in the mouse. Moreover, because of the important role that adhesion molecules play in immune responses in the central nervous system, upregulation of PECAM-1 prior to structural maturity of the BBB may suggest that the development of an ’’immune BBB” manifests prior to anatomical closure of the BBB in the mouse.

Relation:

Folia Neuropathologica

Volume:

35

Issue:

3

Start page:

163

End page:

170

Resource type:

Text

Detailed Resource Type:

Article

Format:

pdf

Language:

eng

Language of abstract:

eng ; pol

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open