Title:

DNA protein flow cytometry of dissociated cultures of human anaplastic gliomas. Pattern of proliferation and differentiation and the effect of a new cytostatic drug cladribine (2-CdA)

Subtitle:

Badania zdysocjowanych hodowli ludzkich złośliwych guzów glejopochodnych metodą cytometrii przepływowej DNA-bialko. Obraz proliferacji i różnicowania komórek oraz wpływ nowego leku cytostatycznego cladribine (2-CdA)

Creator:

Grieb, Paweł ; Skierski, Janusz ; Jagielski, Janusz ; Ryba, Mirosław ; Mossakowski, Mirosław Jan (1929–2001)

Publisher:

Medical Publishing House

Place of publishing:

Warsaw

Date issued/created:

1995

Type of object:

Journal/Article

Subject and Keywords:

glial tumors ; cladribine (2-CdA) ; DNA-protein cytometry

Abstract:

A technique of protein-DNA flow cytometry was applied to characterize cell cycling, and to assess the cytotoxicity of cladribine (2-chloro-2’deoxyadenosine) toward seven dissociated cultures of human primary brain tumors (anaplastic astrocytoma and glioblastoma multiforme) grown in vitro from surgical biopsies. Control cytograms were suggestive of that a clonogeneic fraction of the cell population consists mainly of cells with low protein content, which do not require increase in protein content before entering the S phase of the cell cycle. Following 24 or 48 hours exposure to cladribine, 1 nM -1 M, no cytotoxic effect was evident in 4 cultures, whereas in two cases dose-dependent progressive block of the phaseof the cell cycle was noted. In one case a massive cytotoxic effect resulted in disintegration of culture exposed to 100 nM ofthe drug. However, the treatment with cladribine was ineffective in a patient bearing the tumor which was the source forthe last culture, suggesting that cytotoxicity in vitro may not be predictive of clinical response.

Relation:

Folia Neuropathologica

Volume:

33

Issue:

3

Start page:

145

End page:

152

Resource type:

Text

Detailed Resource Type:

Article

Format:

pdf

Language:

eng

Language of abstract:

eng ; pol

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open