Title:

Immunoelectron microscopic demonstration of GABA and glutamate synapses in Mongolian gerbils hippocampus after ischemia

Subtitle:

Zachowanie Gaba- i glutaminergicznych synaps w hipokampie chomików mongolskich w następstwie niedokrwienia mózgu. Mikroskopowo-elektronowe badania immunocytochemiczne

Creator:

Gajkowska, Barbara ; Gadamski, Roman (1933–2015) ; Mossakowski, Mirosław Jan (1929–2001)

Publisher:

Medical Publishing House

Place of publishing:

Warsaw

Date issued/created:

1995

Type of object:

Journal/Article

Subject and Keywords:

forebrain ischemia ; GABA ; glutamate ; immunogold immunocytochemistry

Abstract:

The subcellular distribution of glutamate and GABA in synapses of Mongolian gerbils hippocampus was examined using post-embedding immunogold staining method for electron microscopy. Immunolabelling was performed with 10 nm gold-antibody complexes for glutamate and GABA. The gold particle densities gave reliable information about the relative concentrations of these amino acid neurotransmitters. Our results indicate that ischemia leads to the temporal decrease of GABA-like immunoreactivity in symmetric synapses and a slight enhancement of the level of glutamate-like reactivity in asymmetric ones. The striking finding was a redistribution of glutamate-like immunoreactivity from neurons to glia. This suggests the capacity of glia to metabolize the excess of glutamate after ischemia. The disturbances at the level of neurotransmitters and their possible role in hippocampal neuronal injury were stressed.

Relation:

Folia Neuropathologica

Volume:

33

Issue:

1

Start page:

5

End page:

19

Resource type:

Text

Detailed Resource Type:

Article

Format:

pdf

Language:

eng

Language of abstract:

eng ; pol

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open