Title:

Immunochemical analysis of some proteins in cerebrospinal fluid and serum of patients with ischemic strokes

Subtitle:

Analiza immunochemiczna niektórych białek w płynie mózgowo-rdzeniowym i surowicy krwi chorych z udarem niedokrwiennym

Creator:

Niebrój-Dobosz, Irena ; Rafałowska, Janina ; Łukasiuk, Mirosława ; Pfeffer, Anna ; Mossakowski, Mirosław Jan (1929–2001)

Publisher:

Medical Publishing House

Place of publishing:

Warsaw

Date issued/created:

1994

Type of object:

Journal/Article

Subject and Keywords:

ischemic stroke ; cerebral proteins ; Cerebrospinal Fluid ; blood serum

Abstract:

Immunochemical studies of yy-neuron specific enolase (NSE), parvalbumin (PV), S-100 protein (S-100) and acidic fibrillary glial protein (GFAP) were studied in the cerebrospinal fluid and blood serum in 7 patients with ischemic cerebral stroke, aged 57 to 81 years. Cerebrospinal fluid and the first blood sample were taken on the first or second day of the disease. Further blood samples were taken once a week till the end of patients hospitalization, ending by patients discharge or death. Immunochemical identification of proteins under study were performed with Western-blotting technique. It was found that all proteins studied were present in both cerebrospinal fluid and blood serum on the first two days of the disease in small quantities. The blood content of both NSE and PV increased significantly during the first week of the disease. Both proteins disappeared from the blood serum between the second and fourth disease weeks. S-100 protein and GFAP contents in the blood reached significantly high level within the time interval between secondand fifth disease weeks, and remained at a relatively high level till patients' death. In all cases computed tomography study and/or brain autopsy revealed extensive ischemic foci localized within areas supplied by the middle cerebral artery. No clear-cut correlation between extensiveness of the ischemic cerebral damage and the content of the proteins studied in both cerebrospinal fluid and blood serum was found. However, our data indicate that serial studies of the above proteinsin patients with ischemic stroke may be useful in monitoring the progress of the disease, and occasionally in the prognosis at least in some cases.

Relation:

Folia Neuropathologica

Volume:

32

Issue:

3

Start page:

129

End page:

137

Resource type:

Text

Detailed Resource Type:

Article

Format:

pdf

Language:

eng

Language of abstract:

eng ; pol

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

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