Somaclonal variation in micropropagated Hemerocallis sp. plants determined by phenotype and molecular markers, RAPD and ISSR

Podwyszyńska. Małgorzata; Gabryszewska, Eleonora; Korbin, Małgorzata; Jasiński, Artur

doi:IChB B-84

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Ocena zmienności somaklonalnej liliowców rozmnożonych in vitro na podstawie obserwacji fenotypowych oraz przy użyciu RAPD i ISSR Podwyszyńska. MałgorzataGabryszewska, EleonoraKorbin, MałgorzataJasiński, Artur

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Ocena zmienności somaklonalnej liliowców rozmnożonych in vitro na podstawie obserwacji fenotypowych oraz przy użyciu RAPD i ISSR

Somaclonal variation in micropropagated Hemerocallis sp. plants determined by phenotype and molecular markers, RAPD and ISSR

Podwyszyńska. Małgorzata ; Gabryszewska, Eleonora ; Korbin, Małgorzata ; Jasiński, Artur

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

2010

biotechnologia

The genetic fidelity of the micropropagated daylily plants was evaluated by a phenotypic observation and with the molecular markers, randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) techniques. The plants of nine daylily cultivars propagated in vitro for the period of one to five years and grown outdoors in the years 2002 to 2007 were used in the study. The somaclonal variants occurred within the micropropagated plants of the five genotypes. The somaclones differed from the true-to-type plants in terms of colour and shape of the flowers. Depending on the genotype, the frequency of somaclonal variation (SV) ranged from 1.4% to 100%. The RAPD and ISSR analyses of the selected somaclones confirmed the polymorphism between true-to-type plants and somaclones on the level of DNA.

Biotechnologia, vol.89, 2 (2010)-.

Biblioteka Instytutu Chemii Bioorganicznej PAN

pol

eng

2010

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