The control and eradication of bacterial infections and contaminations in plant tissue culture

Zenkteler, Elżbieta

doi:IChB B-84

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Kontrola i zwalczanie zakażeń i zanieczyszczeń bakteryjnych w kulturach roślinnych in vitro Orlikowska, TeresaSobiczewski, P.Zawadzka, M.Zenkteler, Elżbieta

Object structure

Kontrola i zwalczanie zakażeń i zanieczyszczeń bakteryjnych w kulturach roślinnych in vitro

The control and eradication of bacterial infections and contaminations in plant tissue culture

Orlikowska, Teresa ; Sobiczewski, P. ; Zawadzka, M. ; Zenkteler, Elżbieta

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

2010

prace przeglądowe

biotechnologia

Bacterial contamination is a serious problem in plant tissue culture. In in vitro cultures, bacteria are introduced, with the initial which is most frequently explants, but bacterial contamination can also come from the laboratory environment or from the staff themselves. Exogenous bacteria are easier to deal with, but endogenous bacteria remain problematic. Standard sterilization with ethanol, NaOCl or HgCl2 and with antibiotics can now be enriched with new components (NaDCC, AgN03, nano-silver) or sanitation products (PPM™, ProClin® 300, Biosept 33 SL, Vitrofural®, Dekaben). Some of these can be incorporated into a multiplication and rooting medium for one or more passages, if they are not phytotoxic to the plant explants. A special problem is presented by cryptic or viable but not cultivable bacteria which can be unable to multiply during many passages, but finally be disclosed in mass. The issue is, therefore, to Find and apply tools for detection of selective media and/or molecular markers. The above questions are discussed in the present paper based on the literature and results of our own study.

Biotechnologia, vol.89, 2 (2010)-.