Title:

Cytochrome P-450 metabolites in renal circulation and excretion-interaction with the nitric oxide (NO) system

Creator:

Kompanowska-Jezierska, Elżbieta ; Kuczeriszka, Marta

Contributor:

Laboratory of Renal and Body Fluid Physiology, M. Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw,Poland

Publisher:

Polish Physiological Society

Place of publishing:

Krakow

Date issued/created:

2008

Type of object:

Journal/Article

Subject and Keywords:

Cytochrome p-450 ; NOS ; Sodium intake ; intrarenal circulation ; Renal excretion

Abstract:

The role of CYP-450 dependent arachidonic acid (AA) metabolites (vasoconstrictor 20-HETE and vasodilator EETs) and NO in control of blood pressure (MABP) and kidney function remains unclear. NO affects the activity of heme-containing enzymes, like CYP-450 related monooxygenases, moreover, their activity depends on Na(+) intake. The focus of this review and underlying studies is on the role of high sodium intake (pro-hypertensive factor) in interrelation between CYP-450 and NOS. The acute vs. chronic non-selective inhibition of CYP-450 AA metabolites (ABT), and selective inhibition of 20-HETE (HET 0016) has also been tested. The renal artery flow (RBF, Transonic probe), medullary blood flow (MBF, laser-Doppler flux), renal excretion, and medullary tissue NO (selective electrode) were measured in male anaesthetized Wistar rats. We conclude that on standard Na(+) intake, opposed effects of 20-HETE and EETs are almost in equilibrium; however, in the renal circulation the vasodilator EETs influence slightly prevails. High sodium intake stimulates NOS, which limits CYP-450 impact on MABP and kidney function. However, this protection disappears after prolonged sodium intake. Long-lasting high sodium intake lowers NO bioavailability and promotes systemic and intrarenal vasoconstrictor activity of 20-HETE. Opposed effects of NO and AA metabolites of CYP-450 on water and solute excretion are also described.

Relation:

Journal of Physiology and Pharmacology

Volume:

59

Issue:

Suppl 9

Start page:

137

End page:

149

Resource type:

Text

Detailed Resource Type:

Article : review article

Format:

text/xml

Language:

eng

Language of abstract:

eng

Rights:

Rights Reserved - Free Access

Terms of use:

Copyright-protected material. [CC BY 3.0 PL] May be used within the scope specified in Creative Commons Attribution BY 3.0 PL license, full text available at:

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Programme Innovative Economy, 2010-2014, Priority Axis 2. R&D infrastructure

Access:

Open