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Szczepanek, Andrzej ; Płucienniczak, Andrzej
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:Escherichia coli is the most useful bacterial species applied to genetic engineering in recombinant proteins production process. The supply of many polipeptides whith potential clinical orindustrial use is often limited by their low natural availability. Overexpressed polipeptides mayeither be located in the cytoplasm and periplasm of E. coli or secreted through the cell membraneinto the growth medium. Foreign proteins can be expressed in E. coli cells directly or as fusionproteins with prokaryotic sequences. Frequently, the overexpressed proteins acumulate in thebacterial cytoplasm or periplasm in the form of insoluble inclusion bodies.This review considers isolation, purification, solubilization and renaturation of recombinantproteins from E. coli cells, whieh is still a serious methodological and technical problem.
Relation:Biotechnologia, vol.27, 4 (1994)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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