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Ekspresja genów heterologicznych w Escherichia coli na modelowo ujętym przykładzie interferonu gamma
Subtitle:Ekspresja genów heterologicznych w Escherichia coli na modelowo ujętym przykładzie interferonu gamma
Creator: Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Type of object: Subject and Keywords: Abstract:Interferon gamma is a molecule which may serve as a good object for studying and explainingthe role of expression limiting conditions.Three levels of limiting factors modifications, i. e. operon choice and structure, changes inthe secondary mRNA structure, and the influence of post-translational modulation processes aredescribed. The relationship between the operon stucture and expression level for other heterologous proteins is compared with that of IFN-gamma. In particular, the shape of ribosome bindingsite (RBS) region and its affected different nucleotide substitutions are presented.The rule of N-terminal and C-terminal end changes for protein stability and inclusion bodyformation is also shown.
Relation:Biotechnologia, vol.37, 2 (1997)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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