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Sposoby identyfikacji markerów RAPD w rośiinach
Subtitle:Sposoby identyfikacji markerów RAPD w rośiinach
Creator: Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:The random amplified polymorphic DNA (RAPD) method is based on random amplificationof DNA fragments, via PCR, using short primers of arbitrary sequence. RAPD markers have beenapplied to construct linkage maps, to assess genetic diversity, to study taxonomic relationships,and to tag disease resistance genes in plants. RAPD markers linked to a resistance gene canbe identified using bulked segregant analysis (BSA), recombinant inbred lines (RlLs) or nearisogenic lines (NlLs). More reliable and specific PCR-based markers known as sequence-characterized amplified region (SCAR) and allele-specific associated primer (ASAP) were developed. Thereare several examples of the application of these DNA marker systems in marker-assisted plantbreeding.
Relation:Biotechnologia, vol.44, 1 (1999)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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