Title:

Enzymatic Hydrolysis of Waste Fats

Subtitle:

Enzymatic Hydrolysis of Waste Fats

Creator:

Antczak, Tadeusz ; Krystynowicz, Alina ; Galas, Edward

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2000

Subject and Keywords:

biotechnology

Abstract:

The intracellular lipases of Mucor circinelloides and Mucor racemosus immobilised in situ as well as the extracellular lipase of Rhizopus nigricans in solubleform were applied to enzymatic hydrolysis ofwaste fats from oil and meat industry, waste sludge of fats from municipal and industrial sewage-treatmentplants and waste water containing microemulsion of fats. The reaction of hydrolysis of plant and animal waste fats was carried out by two methods. Thefirst one was a partial hydrolysis of acylglycerols to obtain mono- and diacylglycerols. The second variant was complete hydrolysis of fats to obtain glyceroland free fatty acids. It has been demonstrated that lipase of Rhizopus nigricanspartly hydrolysed waste fats. The products of hydrolysis contained 35-45% mono- and diacylglycerols. These products may be used as emulsifier in the hydrolysis of fats. The yield of hydrolysis of waste fats by immobilised Mucor lipases was from 85 up to over 95%. Waste sludge of fats from municipal and industrial sewage contain 12-62% of free fatty acids. This kind of material was hydrolysed without an emulsifier but with the addition of calcium chloride. Theyield of hydrolysis of acylglycerols was from 45 to 76%. Tne yield of hydrolysis ofmicroemulsion of fats in waste water by immobilised Mucor lipases was from 80up to over 95%. As the hydrolysis products contained free fatty acids, mono- anddiacylglycerols, it was easy to make emulsions which could be utilised in anaerobic-aerobic processes.

Relation:

Biotechnologia, vol.49, 2 (2000)-.

Volume:

49

Issue:

2

Start page:

120

End page:

130

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-45

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open