Title:

Suspension cultures of Pharbitis nil

Subtitle:

Suspension cultures of Pharbitis nil

Creator:

Szyp, Iwona ; Tretyn, Andrzej

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2001

Subject and Keywords:

biotechnology

Abstract:

Suspension cultures are more suitable for physiological, biochemical andmolecular investigations than callus cultures grown on solid media, because theformer provide more homogeneous system than the latter ones. A large numberof plants were found suitable for establishing cell suspension cultures. In thisstudy we describe the obtaining of cell suspension cultures derived from callusinduced from cotyledons of Pharbitis nil. Explants isolated from plants grownunder inductive and non-inductive conditions were cultured on MS basal medium containing various concentrations and combinations ofgrowth regulators.To initiate the cell suspension culture, small clumps of friable callus obtainedfrom cotyledons were suspended in liquid callusing medium. An initial inoculumdensity was 2 x lO'^cells/ml. Every 20 days the cultures were transferred to afresh medium. The cell number in suspension was determined by direct microscopic counting with haemocytometr. The cell suspension cultures containedboth single cells and small cell aggregates

Relation:

Biotechnologia, vol.52, 1 (2001)-.

Volume:

52

Issue:

1

Start page:

127

End page:

130

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-47

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open