Heteroplasmy in plants

Wołoszyńska, Magdalena; Oziemlak, Aneta; Jańska, Hanna

doi:0860-7796

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Heteroplazmia u roślin Wołoszyńska, MagdalenaOziemlak, AnetaJańska, Hanna

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Heteroplazmia u roślin

Heteroplasmy in plants

Wołoszyńska, Magdalena ; Oziemlak, Aneta ; Jańska, Hanna

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

2001

biotechnologia

Heteroplasmy is the state characterised by the presence of more than onetype of mitochondrial or chloroplast genome in one organism. Infrequent recombinations across short repeated sequences often lead to heteroplasmy inhigher plant mitochondria. Different Pbaseolus species have been examined inorder to understand the dynamics of heteroplasmy originating through recombination mediated by the 314 bp repeated sequence. Two techniques were applied to detect heteroplasmy: Southern hybridization with prolonged autoradiography and PCR amplification followed by hybridization or reamplification.In all examined genomes the four recombination forms were detected. However,these forms do not occur in the same relative amounts. Moreover, in the examined genomes different recombination forms exist at the predominant/substoichiometric level, but always only two ofthem are predominant. Based on theseresults and the previous data, we suggest that the changes in the heteroplasmicpopulation of mitochondrial molecules in plants may offer the source of geneticvariation in the course of evolution.

Biotechnologia, vol.52, 1 (2001)-.