Subtitle:

Development of an automated yeast identification system by the application of PCR technique: a comparison of Inter-LINE PCR with reference methods

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In the Inter-LINE (IL) PCR method, oligonucleotides GF, GR and 01, whichoriginated from mammalian cells led to highly reproducible patterns of amplified template DNA, based on the consensus of LlNE-sequences. These were usedfor the genomic fingerprinting of about 80 strains of yeast, consisting of 30 species from 13 genera. The IL-PCR technique using the above primers is describedand compared to reference methods such as Arbitrarily Primed-PCR and PulseField Gel Electrophoresis (PFGE).A comparison of the two PCR variants was performed using suitable numbers of digitized PCR-fmgerprints. A database for an automated yeast identification system is proposed.

Relation:

Biotechnologia, vol.55, 4 (2001)-.

Volume:

55

Issue:

4

Start page:

122

End page:

134

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138601 ; IChB B-50

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open