Title:

Green fluorescent protein (GFP) - an ecological transformation marker and a tool for observing processes in the living cell

Subtitle:

Green fluorescent protein (GFP) - an ecological transformation marker and a tool for observing processes in the living cell

Creator:

Śliwińska, Elwira

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2002

Subject and Keywords:

biotechnology

Abstract:

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria hasbeen extensively used as a marker of gene expression and protein targeting inintact cells and organisms. Since GFP has no toxic effect on cells and it is easy todetect, it can replace controversial transformation markers such as those for antibiotic or herbicide resistance. The protein can also be used to detect dynamicresponses of signal transduction pathways, subcellular localisation of chimericproteins, and transfection efficiency. It allows visualisation of the organellesand observation of many important cellular processes.

Relation:

Biotechnologia, vol.56, 1 (2002)-.

Volume:

56

Issue:

1

Start page:

129

End page:

135

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-52

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open