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Phage display - a new way of selecting proteins with predefined specificities
Subtitle:Phage display - a new way of selecting proteins with predefined specificities
Creator:Kwaśnikowski, Piotr ; Talarek, Joanna ; Czerwiński, Marcin ; Markiewicz, Wojciech T.
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:The display of diverse repertoires of proteins on the surface of filamentousbacteriophage offeres a new way of selecting proteins with predefinedspecificities. The affinity selection process can be carried out due to unique feature of the phage expression system, the direct physical linkage between genotype and phenotype of displayed proteins. Phage dysplayed libraries of proteinshave been sucessfully used in a number of applications, including selection ofmonoclonal antibodies, receptor ligands and enzyme inhibitors, cDNA expression, epitope mapping, studies on protein-protein and protein-nucleic acids interactions. The phage dislay system is also a very decent tool to perform in vitroevolution of protein properties. This approach is based on construction andscreening of reprtoires of mutagenized proteins or protein domains and affinityselection of molecules with most desirable features.
Relation:Biotechnologia, vol.56, 1 (2002)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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