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Badanie kinetyki enzymatycznej hydrolizy tanin
Subtitle:Kinetic study of enzymatic hydrolysis of tannins
Creator:Pohorecki, Ryszard ; Galwas-Zakrzewska, Magdalena
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:Tannins are present in many plant foods. Tannase (EC 3.1.1.20) catalyses hydrolysis of ester bonds in hydrolysable gallotannins releasing glucose and gallicacid. Enzyme was produced by Penicillium chrysogenum cultured in Czapek-Doxmedium containing tannic acid. The tannic acid was used as a substrate in theenzymatic reactions. The product inhibits the enzymatic reaction. It was determined that inhibition was of the competitive type. The mathematical model(Eg. 7) and kinetic constancy describing the process were determined:Km = 0,53-10 ‘'|mol/dm3), Rm = 1,2610-3 (mol/dm^minj, Kp=2,04-10-'* (mol/dm^j.The results obtained from the model well agree with those obtained experimentally.
Relation:Biotechnologia, vol.57, 2 (2002)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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