Tytuł:

The influence of methylene blue and methylene green on the effectiveness of photooxidation of porcine collagen

Inny tytuł:

The influence of methylene blue and methylene green on the effectiveness of photooxidation of porcine collagen

Twórca:

Koźma, Ewa M. ; Głowacki, Andrzej ; Olczyk, Krystyna ; Wisowski, Grzegorz ; Olczyk, Paweł ; Jura-Półtorak, Agnieszka ; Nawrat, Zbigniew

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

2003

Temat i słowa kluczowe:

biotechnology

Abstrakt:

Photooxidation is a method of tissue fixation in the construction ofbioprostheses. This process leads to the tissue protein crosslinking under lightand dye. In the present study, the influence of methylene blue and methylenegreen on the effectiveness of pulmonary valve and aorta collagen modificationwas evaluated on the basis of photooxidized tissues sensitivity to pepsin digestion. The components of tissue hydrolysates were characterized electrophoretically. The tissues photooxidized in the presence of employing dyes showed increased resistance to enzymatic degradation in comparison to the unfixed ones.However, methylene green was more effective in valve and aorta collagencrosslinking. Hydrolysates of the tissues fixed in the presence of mentioned dyedisplayed decrease in collagen a chains and their aggregates’ content.

Czasopismo/Seria/cykl:

Biotechnologia, vol.61, 2 (2003)-.

Tom:

61

Zeszyt:

2

Strona pocz.:

290

Strona końc.:

302

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; IChB B-57

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open