Title:

The transgenes are expressed with different level in plants

Subtitle:

The transgenes are expressed with different level in plants

Creator:

Yin, Zhimin ; Malepszy, Stefan

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Date issued/created:

2003

Subject and Keywords:

biotechnologia

Abstract:

Variable expression for the same transgene construct has been documentedin various plant species, regardless of the type of transgene. This phenomenonand the factors influencing it are reviewed. A variability in the transgene expression level was found to exist between and/or within independently derivedlines, different lines of clonal replicates, siblings ofthe same line, a single plant,a single leaf as well as the same cell. Both the transgene dependent and the recipient dependent factors have been proposed to account for this phenomenon,the transgene dependent factors of which include transgene construct fidelity,T-DNA integration pattern, T-DNA copy number, promoter activity and the effect of nuclear matrix attachment region. In addition, different forms of epigenetic, homology-dependent gene silencing also contribute to the unstable expression ofthe identical transgene. The recipient dependent factors include position effect, ploidy level, genetic background, homozygosity, and developmental stage. Furthermore, environmental factors such as light intensity, temperature, field growth conditions and the season have been shown to modulate thelevels of transgene expression. The approaches to stabilize the transgene expression were also discussed.

Relation:

Biotechnologia, vol.61, 2 (2003)-.

Volume:

61

Issue:

2

Start page:

236

End page:

260

Resource type:

Tekst

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-57

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty