Title:

Preparation of PGIP-based genetic construct for modification of strawberry genome to obtain genotypes resistant to grey mould

Subtitle:

Preparation of PGIP-based genetic construct for modification of strawberry genome to obtain genotypes resistant to grey mould

Creator:

Korbin, Małgorzata ; Majka, Jolanta ; Gruchała, Agnieszka ; Żurawicz, Edward

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2004

Subject and Keywords:

biotechnology

Abstract:

Polygalacturonase inhibiting protein (PGIP) cDNA was isolated using RT-PCR from fruit of apple ‘Golden Delicious’ which was previously wounded and inoculated with Botrytis cinerea. The sequence of the cDNA showed high level of homology with the published sequence of PGIP-cDNA from apple and pear (99,7 and 97,3%) and low similarity with the sequence of cDNA from tomato (51,5%). The obtained cDNA was cloned and used for the binary construct creation. The introduction of the pBIN19-PGlP construct into strawberry plant genome seems to be a promising strategy to obtain a genotype resistant to B. cinerea, causal agent of grey mould - the most economically important fungal disease of this species.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

22

End page:

28

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open