@misc{Rusin_Marek_Gene_1992, author={Rusin, Marek and Szala, Stanisław}, volume={17}, number={2}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.17, 2 (1992)-.}, howpublished={online}, year={1992}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={Genes expressed in temporally, environmentally or tissue specific way can be cloned using subtractivehybridization. It involves hybridization of single stranded cDNA from one cell type with mollar excess ofpolyA+RNAfrom another cell type. Unhybridized, cell specific cDNA is subsequently used or as subtractiveprobe or as material for construction of subtractive cDNA library. Modifications of this basic strategy arealso discussed.}, title={Gene cloning using subtractive hybridization}, type={Text}, URL={http://rcin.org.pl./Content/163606/PDF/POZN271_197358_biotechnologia-1992-no2-rusin.pdf}, keywords={biotechnology}, }