@misc{Oleszczuk_S._In_2000,
 author={Oleszczuk, S. and Zimny, J.},
 volume={49},
 number={2},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.49, 2 (2000)-.},
 howpublished={online},
 year={2000},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={The development of microspore culture methods in the Poaceae family hasreceived considerable attention in recent years. Isolated microspore culture canbe induced in vitro to switch their development from gametophytic to a sporophytic patway, resulting in embryoid or callus formation. Different stresseslike cold or heat shock and nitrogen starvation have been identified as the major trigger in inducing microspore embryogenesis. Microspore culture appearsto be a promising tool for future production of double haploids in cereals. Isolated microspore culture has several advantages over anther culture in geneticmanipulation and haploid study, such as: direct observation of microspore development, unique possibility to study plant embryogenesis, easier in vitro selection and mutation, easier transformation of single cells. Moreover, isolatedmicrospores are the most efficient way of double haploid regeneration. Manyfactors such as genotypes, physiological status of donor plants, stage of microspores development, pretreatment of anthers or spikes, method of microsporesisolation, culture media, nurse culture and culture conditions, have a great influence on microspore culture. These and other problems concerning in vitroculture of isolated microspore are discussed in this review.},
 title={In Vitro Culture of Cereal Microspores},
 type={Text},
 URL={http://rcin.org.pl./Content/141527/PDF/POZN271_176830_biotechnologia-2000-no2-oleszczuk.pdf},
 keywords={biotechnology},
}